方法通过基因芯片分析法对比找出伊马替尼耐药株SUP-B15/R与敏感株SUP-B15/S之间表达差异的基因
分别采用q-PCR和Western-Blot进一步验证SLC2A5及其编码蛋白葡萄糖转运体5（Glut5）在SUP-B15/R与SUP-B15/S之间表达的差异
探究Glut5表达增加在SUP-B15细胞伊马替尼耐药中的作用
） 摘要： 目的采用Ph+急性淋巴细胞白血病（Ph+ALL）伊马替尼耐药细胞株SUP-B15/R研究伊马替尼耐药的可能机制
龚玉萍
Glut5在Ph+急性淋巴细胞白血病伊马替尼耐药中的机制研究[EB/OL]
北京：中国科技论文在线
采用MTT实验检测果糖对SUP-B15/S细胞伊马替尼敏感性的影响
关键词： Ph+急性淋巴细胞白血病; 伊马替尼耐药; 葡萄糖转运体5; 果糖 YAN Tianyou
与细胞代谢相关的SLC2A5基因在SUP-B15/R中高表达
而果糖处理后SUP-B15/S细胞对伊马替尼的敏感性下降
） Abstract： Objective To explore the possible roles of Glucose transport 5 (Glut5) in imatinib resistancThe Roles of Glut5 in Imatinib Resistance in the Ph+ Acute Lymphoblastic Leukemia Celle in the Ph+ acute lymphoblastic leukemia cell (Ph+ ALL)
Methods TheThe Roles of Glut5 in Imatinib Resistance in the Ph+ Acute Lymphoblastic Leukemia Cell gene chip technique was used to detect different gene expression between Ph+ ALL cell line SUP-B15/R (Imatinib resistant cell line) and SUP-B15/S (Imatinib sensitive cell line), the gene of SLC2A5 and its coded protein Glut5 was screened out and was reconfirmed by q-PCR and Western-Blot assay
The Imatinib IC50 to SUP-B15/S cells with or without fructose treatment was further detected by MTT assay, simultaneously signal pathway gene was detected by q-PCR assay
Results Metabolism related gene SLC2A5 was screened out from the overexpression genes in SUP-B15/R cells detected by gene chip
q-PCR and Western-Blot assay confirmed the high expression of SLC2A5 gene and its Glut5 protein
IC50 values of Imatinib to SUP-B15/S cells after treated with 25μmol/L fructose increased from 44
50±2
375 μmol/L to 64
71±1
685 μmol/L, in the meanwhile, PI3K and Akt mRNA level also increased in fructose treated SUP-B15/S cells compared to the control
Conclusions High expression of Glut5 in SUP-B15/R cells leads to increased fructose absorption, and further activates PI3K/AKT pathway which is suppressed by Imatinib, finally causes SUP-B15/R cell resistant to Imatinib
Keywords： Ph+ acute lymphoblastic leukemia; Imatinib resistance; Glucose transport 5; fructose
下载PDF阅读器 PDF全文下载： 初稿 （ 0 ） 作者简介： 严天友(1991
11-),男,白血病基础研究 通信联系人： 龚玉萍(1964
10-),女,博导,白血病基础研究 【收录情况】 中国科技论文在线： 严天友
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