3种富勒烯衍生物对HeLa细胞内GSH水平的影响及机理探讨[EB/OL]
北京：中国科技论文在线
） 摘要： 采用二加成亚甲基富勒烯[60]二膦酸四乙酯 (BFDP)、二加成富勒烯[60]丙二酸 (DMA C60)和富勒醇(fullerol) 3种富勒烯衍生物研究富勒烯衍生物的结构与光诱导细胞内GSH耗竭的关系．通过DTNB法测定它们对人HeLa细胞内GSH的影响．研究发现
BFDP诱导细胞凋亡、耗竭GSH的机理主要是与BFDP活性氧产生有关
在光照条件下3种化合物（5 μmol/L）中BFDP对HeLa内GSH耗竭作用最强
） Abstract： Three fullerene derivatives including a bisadduct of tetraethyl methano[60] fullerenediphosphona- te (BFDP)、a dimalonic acid[60]fullerene ((DMA C60)and fullerol were used to study the relationship between their photo-induced GSH depletion and structures
Their effects on the GSH of human HeLa cells were determined by DTNB assay
We found that BFDP（5 μmol/L）was the most effective on GSH depletion among them
And the longer BFDP incubated with cells after irradiation, the higher the rate of GSH depletion and cell apoptosis
In addition, reactive oxygen species (ROS) scavengers were able to partly prevent GSH depletion
The results indicated that the photo-induced cell apoptosis and GSH depletion of BFDP was mainly related to ROS production
Keywords： fullerene derivatives, HeLa cell, GSH depletion 下载PDF阅读器 PDF全文下载： 修改稿 1（ 67 ） 初稿 （ 170 ） 作者简介： 通信联系人： 【收录情况】 中国科技论文在线： 银亚冰
乔新歌 （ 北京理工大学
HeLa细胞
关键词： 富勒烯衍生物
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且GSH的耗竭率和凋亡率随孵育时间的延长而增大．活性氧清除剂可以部分抑制BFDP的作用
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