总览 评价 REN Hao * （ Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University
） Abstract： The cell cycle inhibitor flavopiridol was previously shown in animal models to improve SCI recovery
However, the systematic dosage of flavopiridol is with side effects and the mechanism is not clear
This study aims to develop a strategy for local delivery of flavopiridol and investigate its mechanisms of function as well
Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) was used for the sustained delivery of flavopiridol
The spinal cords were right-hemisectioned and NPs were administered into the injury site
Transparent spinal cord technology was applied for the three-dimensional observation of the anterograde tracing
The results showed that flavopiridol NPs had a susteined release of up to 3 days in vitro
Flavopiridol NPs significantly decreased inflammatory factor synthesis of astrocytes, including tnf-alpha, il-1beta, and il-6, while the il-10 expression was elevated
In-vivo study demonstrated that flavopiridol NPs decreased inflammatory expression and glial scarring, and facilitated neuronal survival and neural transmitting
Cavitation volume was decreased by proximately 90%
Administration of flavopiridol NPs also improved the motor recovery of injured animals
These findings illustrated that local delivery of flavopiridol in PLGA NPs improved SCI recovery through inhibiting astrocyte growth and inflammatory factor synthesis
Keywords： spinal surgery; cell activation; nanoparticle; astrocyte; inflammation; drug release 下载PDF阅读器 PDF全文下载： 初稿 （ 56 ） 作者简介： REN Hao (1989-), male, PhD candidate, stem cell and regenerative medicine 通信联系人： 【收录情况】 中国科技论文在线： REN Hao
Local Delivery of Flavopiridol in PLGA Nanoparticles Improves the Repair of Spinal Cord Injury by Inhibiting Astrocyte Growth and Inflammatory Factor Synthesis[EB/OL]
北京：中国科技论文在线
） 摘要： The cell cycle inhibitor flavopiridol was previously shown in animal models to improve SCI recovery
However, the systematic dosage of flavopiridol is with side effects and the mechanism is not clear
This study aims to develop a strategy for local delivery of flavopiridol and investigate its mechanisms of function as well
Poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) was used for the sustained delivery of flavopiridol
The spinal cords were right-hemisectioned and NPs were administered into the injury site
Transparent spinal cord technology was applied for the three-dimensional observation of the anterograde tracing
The results showed that flavopiridol NPs had a susteined release of up to 3 days in vitro
Flavopiridol NPs significantly decreased inflammatory factor synthesis of astrocytes, including tnf-alpha, il-1beta, and il-6, while the il-10 expression was elevated
In-vivo study demonstrated that flavopiridol NPs decreased inflammatory expression and glial scarring, and facilitated neuronal survival and neural transmitting
Cavitation volume was decreased by proximately 90%
Administration of flavopiridol NPs also improved the motor recovery of injured animals
These findings illustrated that local delivery of flavopiridol in PLGA NPs improved SCI recovery through inhibiting astrocyte growth and inflammatory factor synthesis
关键词： spinal surgery; cell activation; nanoparticle; astrocyte; inflammation; drug release REN Hao * （ Center for Stem Cell and Tissue Engineering, School of Medicine, Zhejiang University
[2014-05-02]
http://www
paper
edu
cn/releasepaper/content/201405-2
