利用PCR技术克隆了苏云芽孢杆菌的Vip3A基因和烟草的EF1α启动子
杨迎伍等
Vip3A基因植物表达载体的构建及烟草的遗传转化[EB/OL]
北京:中国科技论文在线
构建了分别由组成型CaMV35S启动子和花特异表达的EF1α启动子驱动Vip3A基因的植物表达载体pBIVip3A和pBIEFVip3A
EF1α启动子
) 摘要: 为了研究Vip3A基因在转基因抗虫植物中的应用
Vip3A gene of Baciius thuringiensis and EF1α promoter of tobacco were cloned by PCR
Two plant expression vectors pBIVip3A and pBIEFVip3A were constructed,in which the Vip3A gene driven by the constitutive promoter CaMV35S and petal tissue–specific promoter EF1α respectively,and tobaccos were tansformed by Agrobacterium containing the constructed vectors
The transgenic lines were selected by PCR detestion
Keywords: Vip3A gene, EF1α promoter, Bacillus thuringiensis, construction of expression vector, tobacco 下载PDF阅读器 PDF全文下载: 初稿 ( 202 ) 作者简介: 通信联系人: 【收录情况】 中国科技论文在线: 马作江
并通过农杆菌介导的方法对烟草进行了遗传转化