） Abstract： Objective: The present study was undertaken to evaluate anti-proliferative and pro-Apoptotic activities of allicin—a plant derived organics sulfur compounds (OSC) in murine T-lymphocytes (EL-4) and try to determine its molecular mechanism of inducing apoptosis
Methods: Trypan blue dye exclusion method was used to determine cells concentration, the apoptotic rate and morphologic alteration of nuclei were measured by Hoechst 33258 fluorescent dye
DNA spallation was determined by using TUNEL kit
Protein expression of Bax, Bcl-2, cythrome C, Caspase-3, -12 were detected by Immunohistochemistry (IHC)
Fluorescent microscope was used to determine the change of mitchrodria membrane potential (MMP)
Result: Allicin is effective in inhibiting the proliferation of EL-4 cells in vitro in concentration-dependent manner, induces the formation of apoptotic bodies, nuclear condendensation, DNA spallation, and it even activates the expression of caspase-3, -12, cytochrome C, upregulates the ratio of the heterodimer Bax-Bcl-2 and induces MMP decrease
Conclusions: Allicin may induce apioptosis in EL-4 cells with a time- and concentration-dependent manner, in which the mitochnodria pathway may play a central role
Keywords： allicin, EL4 cell, anti-tumor, apoptosis, MMP 下载PDF阅读器 PDF全文下载： 初稿 （ 205 ） 作者简介： 通信联系人： 【收录情况】 中国科技论文在线： 曹志恒
） 摘要： Objective: The present study was undertaken to evaluate anti-proliferative and pro-Apoptotic activities of allicin—a plant derived organics sulfur compounds (OSC) in murine T-lymphocytes (EL-4) and try to determine its molecular mechanism of inducing apoptosis
Methods: Trypan blue dye exclusion method was used to determine cells concentration, the apoptotic rate and morphologic alteration of nuclei were measured by Hoechst 33258 fluorescent dye
DNA spallation was determined by using TUNEL kit
Protein expression of Bax, Bcl-2, cythrome C, Caspase-3, -12 were detected by Immunohistochemistry (IHC)
Fluorescent microscope was used to determine the change of mitchrodria membrane potential (MMP)
Result: Allicin is effective in inhibiting the proliferation of EL-4 cells in vitro in concentration-dependent manner, induces the formation of apoptotic bodies, nuclear condendensation, DNA spallation, and it even activates the expression of caspase-3, -12, cytochrome C, upregulates the ratio of the heterodimer Bax-Bcl-2 and induces MMP decrease
Conclusions: Allicin may induce apioptosis in EL-4 cells with a time- and concentration-dependent manner, in which the mitochnodria pathway may play a central role
关键词： allicin, EL4 cell, anti-tumor, apoptosis, MMP Zhiheng Cao
王子仁
大蒜素诱导体外培养的小鼠淋巴细胞（EL-4）凋亡的研究[EB/OL]
北京：中国科技论文在线
王子仁 * （ 兰州大学生命科学学院
总览 评价 曹志恒
Ziren Wang * （ School of Life Sciences, Lanzhou University
[2008-02-02]
http://www
paper
edu
cn/releasepaper/content/200802-27
