Obestatin（10-13 ~ 10-7 mol/L）单独作用于MES23
5细胞
与200 μmol/L MPP+共孵育MES23
5细胞12或24小时
宋宁等
Obestatin在多巴胺能MES23
5细胞系中通过增殖而非抗凋亡发挥保护作用[EB/OL]
北京：中国科技论文在线
结果 MPP+导致MES23
5多巴胺能细胞存活率降低
10-13 mol/L）处理细胞12小时后PCNA蛋白表达增多
Obestatin（10-13 ~ 10-7 mol/L）预处理能够拮抗这一作用
） Abstract： In this paper Objective Obestatin is an endogenous peptide sharing the same peptide precursor with ghrelin
We aimed to investigate whether and how obestatin could exert neuroprotective effects on MES23
5 dopaminergic cells against 1-methyl-4-phenylpyridinium (MPP+)-induced neurotoxicity
Methods MES23
5 cells were pretreated with obestatin (10-13 ~ 10-6 mol/L) for 20 min prior to 200 μmol/L MPP+ incubation for 12 or 24 hrs, or treated with different doses of obestatin (10-13 ~ 10-7 mol/L) for 0, 6, 12, 24 hrs, respectively
Methyl thiazolyl tetrazolium (MTT) assay was used to measure cell viability
Flow cytometry was used to measure the caspase-3 activity and the mitochondrial transmembrane potential
Proliferating cell nuclear antigen (PCNA) protein levels were determined by western blotting
Results Obestatin (10-13 ~ 10-7 mol/L) pretreatment could block or even reverse MPP+-induced reduction of cell viability in MES23
5 cells
Solely obestatin treatment increased cell viability
However, it had no effects on MPP+-induced mitochondrial transmembrane potential collapse and caspase-3 activation
Elevated PCNA levels were observed with 10-7, 10-9, 10-11 and 10-13 mol/L obestatin treatment for 12 hrs
Conclusion The protective effects of obestatin against MPP+ in MES23
5 cells were due to its proliferative rather than anti-apoptotic effects
Keywords： Obestatin; MPP+; Proliferation; Apoptosis; Dopaminergic cells 下载PDF阅读器 PDF全文下载： 初稿 （ 56 ） 作者简介： SHEN Xiaoli(1978-),Female,Master,Pathogenesis of Parkinsin' disease 通信联系人： JIANG Hong(1973-),Female,Professor,Pathogenesis of Parkinsin' disease 【收录情况】 中国科技论文在线： 沈晓丽
） 摘要： 目的 探讨obestatin（与ghrelin来自同一前体）对MPP+诱导的MES23
