卤醇脱卤酶的共价固定化研究[EB/OL]
北京：中国科技论文在线
） 摘要： 采用环氧树脂ES-103B对卤醇脱卤酶进行了共价固定化
主要研究方向：酶工程与生物催化 【收录情况】 中国科技论文在线： 顾恺
生物催化 通信联系人： 邹树平（1980-）,男
卤醇脱卤酶 GU Kai
最佳固定化条件为：吸附温度为25 ℃
对最优固定化条件及固定化酶的操作稳定性进行了研究
the sodium phosphate buffer is selected as the immobilization buffer and the pH value is 7
0
The concentration of initial enzyme is 0
5 mg/mL and the alkaline treatment time is 24 h
Under the optimized conditions, the recovery activity of enzyme and the immobilization yield reach 74
5% and 94
2%, respectively
And the specific activity is 518
6 U/g
The immobilized enzyme is successfully operated for 15 batches, the yield still remained above 91%, which has a great potential for industrialization
Keywords： Biochemistry and molecular biology; immobilization; epoxy resin; halohydrin dehalogenase 下载PDF阅读器 PDF全文下载： 初稿 （ 0 ） 作者简介： 顾恺（1990-）
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