） Abstract： The 14-3-3 protein is a key player in signal transduction processes in various species of animals and plants
Here, we cloned and expressed the 14-3-3 of Schistosoma japonicum (Sj14-3-3, Chinese mainland strain) in pET28a/E
coli
And the protein can be strongly recognized by polyclonal anti-14-3-3εand sera from animals infected with S
japonicum
Monoclonal antibodies against Sj14-3-3 were raised using a highly specific, affinity-purified protein
The antibodies preparation by ammonium sulfate precipitation was employed for the localization of the native 14-3-3 protein in the parasite by immunofluorescence microscopy
The results demonstrate a wide distribution of this protein in tegument, subtegument, muscle and parenchyma of male, female adult parasites and 15-day-old schistosomulum
The potential use of this protein or its DNA as a vaccine candidate against infection with S
japonicum in BALB/c mice and the recombinant Sj14-3-3 (rSj14-3-3) antigen for diagnosis of schistosomiasis was evaluated
Challenge infection with S
japonicum, immunization with rSj14-3-3, rSjGST, and rSj14-3-3+rSjGST proteins using Freund’s adjuvant or Mycobacterium tuberculosis (Mtb) low molecular peptide as adjuvant respectively, led to 26
0-32
2％ protection, as determined by reduction of adult worm burden; 50
4-58
6％ protection as determined by reduction of the eggs in liver tissue
The mice were directly inoculated with rSj14-3-3 and rSj14-3-3+rSjGST cDNAs through quadriceps femoris muscle for immunization
As the consequence, the worm reduction was found to be 34
20％ in rSj14-3-3 group, 32
40％in rSj14-3-3+rSjGST group; the number of eggs in liver tissue was reduced by 50
74％ and 55
23％, respectively
Indirect ELISA suggested that the rSj14-3-3 antigen is highly sensitive and specific for the diagnosis of schitosomiasis
Keywords： Schistosome;recombinant vaccine;immunodiagnosis 下载PDF阅读器 PDF全文下载： 初稿 （ 557 ） 作者简介： 通信联系人： 【收录情况】 中国科技论文在线： 刘庆中
） 摘要： The 14-3-3 protein is a key player in signal transduction processes in various species of animals and plants
Here, we cloned and expressed the 14-3-3 of Schistosoma japonicum (Sj14-3-3, Chinese mainland strain) in pET28a/E
coli
And the protein can be strongly recognized by polyclonal anti-14-3-3εand sera from animals infected with S
japonicum
Monoclonal antibodies against Sj14-3-3 were raised using a highly specific, affinity-purified protein
The antibodies preparation by ammonium sulfate precipitation was employed for the localization of the native 14-3-3 protein in the parasite by immunofluorescence microscopy
The results demonstrate a wide distribution of this protein in tegument, subtegument, muscle and parenchyma of male, female adult parasites and 15-day-old schistosomulum
The potential use of this protein or its DNA as a vaccine candidate against infection with S
japonicum in BALB/c mice and the recombinant Sj14-3-3 (rSj14-3-3) antigen for diagnosis of schistosomiasis was evaluated
Challenge infection with S
japonicum, immunization with rSj14-3-3, rSjGST, and rSj14-3-3+rSjGST proteins using Freund’s adjuvant or Mycobacterium tuberculosis (Mtb) low molecular peptide as adjuvant respectively, led to 26
0-32
2％ protection, as determined by reduction of adult worm burden; 50
4-58
6％ protection as determined by reduction of the eggs in liver tissue
The mice were directly inoculated with rSj14-3-3 and rSj14-3-3+rSjGST cDNAs through quadriceps femoris muscle for immunization
As the consequence, the worm reduction was found to be 34
20％ in rSj14-3-3 group, 32
40％in rSj14-3-3+rSjGST group; the number of eggs in liver tissue was reduced by 50
74％ and 55
23％, respectively
Indirect ELISA suggested that the rSj14-3-3 antigen is highly sensitive and specific for the diagnosis of schitosomiasis
关键词： 血吸虫
沈继龙 *
沈继龙
Li Defa
Li Feng
李德发等
IMMUNOPROTECTION AND DIAGNOSTIC POTENTIAL OF SIGNALING PROTEIN 14-3-3 OF SCHISTOSOMA JAPONICUM×[EB/OL]
