实验结果：实验组小鼠诱导眼较自身对照眼呈显著近视化发展
洪楠
miR-183/96/182簇在离焦性近视小鼠视网膜中表达差异的研究[EB/OL]
北京：中国科技论文在线
） 摘要： 实验目的：研究离焦性近视小鼠视网膜中miR-183/96/182簇的表达变化
结论：miR-183/96/182簇在离焦性近视小鼠诱导眼的表达明显增高
miRNA-183在诱导眼小鼠表达升高2倍
对照组小鼠不作任何处理
生物信息学分析显示miR-183、miR-96和mmiR-182可能存在多个靶基因
with an experimental period from ages of 4 weeks to 8 weeks
Myopia was monocularly induced over the right eye of the experimental mice using a -15
0 diopter (D) lens, while the contralateral eye and control group were subjected to no disposal
The refraction of mouse eye was tested by streak retinoscopy
Axial parameters were measured with optical coherence tomography system
After 8 weeks, all mice were sacrificed and the eyes were enucleated
The expressions of microRNA-183 (miR-183), microRNA-96 (miR-96) and microRNA-182 (miR-182) in the retina were evaluated by qRT-PCR
Bioinformatics analysis was done using various software and databases
Results: Refraction data demonstrated that the defocusing eyes of the experimental mice achieved a significant myopic shift compared to the contralateral eye, and the defocusing eyes were significantly longer
The expression of miR-183, miR-96 and mmiR-182 in the retina of the lens-induced eyes was almost twice compared with the contralateral eyes
Bioinformatics analysis showed that miR-183, miR-96 and mmiR-182 play crucial role in physiological or pathological status
bConclusion: The over-expression of miR-183、miR-96 and miR-182 suggest MicroRNA may plays an important regulatory role in the development of myopia Keywords： ophthalmology; myopia; mice; microRNA-183
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