2、 Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine
WU Mingxia 4,* （ 1、 Academy of Integrative Medicine, Fujian University of Traditional Chinese Medicine, FuZhou 350122
YE Hongzhi 2,
叶蕻芝 2
） Abstract： Objective: To investigate the molecular mechanisms behind the therapeutic effects of Duhuo Jisheng Decoction (DHJSD) on osteoarthritis (OA)
Methods: A total of 27 two-month-old male Sprague Dawley rats were randomly divided into three groups: the control group (no papain-induced OA; received an equivalent amount of saline only), the model group (papain-induced OA; received an equivalent amount of saline only) and the DHJSD group [papain-induced OA; received a clinical oral dose of DHJSD (9
3g/kg/day)]
After 8 consecutive weeks of treatment, the morphological changes in articular cartilage were observed under an optical microscopy and by transmission electron microscopy (TEM), and the mRNA and protein expression levels of Cyclin D1, CDK4, CDK6, Rb and p16 were measured by RT-PCR and immunohistochemistry, respectively
Results: Treatment with DHJSD significantly improved the arrangement of articular cartilage structure and collagen fibers and reduced cell degeneration compared with the model group
The mRNA and protein expression levels of Cyclin D1, CDK4, CDK6 and Rb in DHJSD-treated group were significantly increased compared to those in the model group, whereas p16 expression was significantly down-regulated
Conclusion: DHJSD treatment promotes chondrocyte proliferation by promoting the G1/S checkpoint transition in the cell cycle and by up-regulating the expression of Cyclin D1, CDK4, CDK6 and Rb and down-regulating the expression of p16 and this may, in part, explain its clinical efficacy in the treatment of osteoarthritis
Keywords： Orthopedics of Chinese Medicine; Duhuo Jisheng Decoction (DHJSD); chondrocyte; proliferation; cell cycle; osteoarthritis 下载PDF阅读器 PDF全文下载： 初稿 （ 209 ） 作者简介： WU Guangwen,(1982-),Male, Research associate, Osteoarthritis research
通信联系人： WU Mingxia(1965-), Female, Professor, Osteoarthritis research 【收录情况】 中国科技论文在线： 吴广文
3、 Academy of Integrative Medicine Biomedical Research Center, Fujian University of Traditional Chinese Medicine
