中药药物血清诱导骨髓间充质干细胞转化为肝细胞的研究[EB/OL]
北京：中国科技论文在线
结论：中药调肝益气通络方具有诱导MSC分化为肝细胞的能力
） 摘要： 目的：探讨骨髓间充质干细胞(MSC s) 体外诱导其分化为肝细胞的可行性及中药调肝益气通络方的有效干预性
2、含药血清诱导MSC分化7,14天后
对照肝细胞生长因子（Hepatocyte Growth Factor ,HGF）诱导MSC向肝细胞的分化若干天后
方法：分离、纯化并鉴定大鼠骨髓MSC,灌胃纤维化模型大鼠以调肝益气通络方后提取药物血清, 用含药血清
14 days; morphology was observed by immunohistochemistry of liver cells iconic protein alpha-fetoprotein (AFP), albumin (ALB), is detected
MTT assay cell growth curve,Results: 1, the concentration of serum containing 1,7,14 days, the number of cells than the conventional training group increased with the dosage, the time is proportional to
2
Serum induced differentiation of MSC positive cells after 7,14 days, AFP 、ALB of high dose group were significantly higher than in HGF group
Conclusion: YiQi Tong Luo Fang has the ability to induce MSC differentiation into liver cells
Keywords： Chinese medicine drug serum; bone mar row mesenchymal stem cells; hepatocytes; Differentiation 下载PDF阅读器 PDF全文下载： 初稿 （ 22 ） 作者简介： 韩海啸（1976-）
