基于冠心病血瘀证心肌细胞能量代谢网络模型的流平衡分析[EB/OL]
北京：中国科技论文在线
） Abstract： objective: to study the characteristics of myocardial cell energy metabolism changes of acute and chronic blood stasis syndrome of coronary artery disease
Methods: (1) using FluxExplorer software platform to build up the coronary heart disease, myocardial cell energy metabolism network model of blood stasis syndrome
(2) application of GC - MS technology and automatic blood gas analyzer from healthy control group, chronic coronary heart disease, coronary heart disease in acute blood stasis of blood stasis syndrome in rats model of coronary arterial blood glucose, fatty acids and blood oxygen content; (3) the results into network model, through the analysis of shadow price under different state of blood stasis syndrome of coronary heart disease characteristic parameters change
Results: (1)three groups of glycolytic, lipidmetabolism and the TCA cycle has beenactivated, but after the netwo-rk operationsmetabolite values were significantly differentin each group showed a healthy control group> group of CHD chronic blood stasis syndrome > group of CHD in acute blood stasis trend
(2) three groups of fatty acids,glucose of energy metabolism in myocardialcells and blood oxygen content and yield of ATPshowed a decreasing trend, and three groups ofkey enzymes of Glycolysis (HK, PFK, PK), key enzymes of the TCA cycle (Cs, Icdh, Od) showed consistent changes
Conclusion: acute blood stasis syndrome of coronary heart disease and coronary blood stasis syndrome of coronary heart disease of different forms are closely relatedto levels of myocardial energy metabolism inthe body
Keywords： Diagnostics of traditional Chinese medicine
方法：①运用FluxExplorer软件平台构建起的冠心病血瘀证心肌细胞能量代谢网络模型
） 摘要： 目的：探讨冠心病急、慢性血瘀证心肌细胞能量代谢的变化特点
结论：冠心病急性血瘀和慢性血瘀不同证候的形成可能与机体内心肌细胞能量代谢的水平密切相关
②应用GC-MS技术和血气自动分析仪从健康对照、冠心病慢性血瘀证、冠心病急性血瘀证三组大鼠模型中取冠状动脉血检测葡萄糖、脂肪酸及血氧含量
通过流平衡分析冠心病急、慢性心血瘀证状态下参数的特点
②三组心肌细胞能量代谢的血糖、脂肪酸和血氧含量及ATP的产量均呈递减的趋势
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