鼠源抗柑橘溃疡病菌Fab抗体库的构建[EB/OL]
北京：中国科技论文在线
结论 本研究成功构建了鼠源抗柑橘溃疡病菌Fab抗体库
） 摘要： 目的 构建鼠源性抗柑橘溃疡病菌( Xanthomonas axonopodis pv
citri , Xac)的噬菌体抗体库
噬菌体Fab抗体库滴度为2
4 ×1011 cfu/ mL
噬菌体抗体库
转化E
coli XL1-Blue后用辅助噬菌体VCSM13超感染构建噬菌体抗体库
结果 构建的Fab抗体库库容为2×106
） Abstract： OBJECTIVE To construct a mouse Fab library against Xanthomonas axonopodis pv
citri through phage- displayed antibodytechnology
METHOD BALB/c mice were immunized with Xanthomonas axonopodis pv
citri
Total RNA was extracted from mice spleens after the fourth immunization
First-srand cDNA was reverse transcribed from total RNA using an oligo (dT)18 primer
Genes of heavy Fd Fragment and light chain of antibodies were amplified by mouse IgG gene-specific primers using the first-srand cDNA as templates
PCR products were cloned into phagmid vector pComb3xss, and transformed into E
col i XL1-Blue competent cells
A mouse combinatorial Fab library was obtained following the infection of help phage VCSM13
RESULTS The size of the Fab library was 2×106 and the recombination rate was 80% as indicated by PCR and endonuclease digestion analysis
The titer of the library was 2
4 ×1011 pfu
CONCLUSION A phage display library of Fab antibody of Mouse following immunization with Xac
was successfully constructed
This research established a solid basis for diagnosis and prevention of Xac
Keywords： Xac;phage display technology;antibody libraryhis paper provides 下载PDF阅读器 PDF全文下载： 初稿 （ 184 ） 作者简介： 通信联系人： 【收录情况】 中国科技论文在线： 王中康
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