蜡状芽孢杆菌(Bacillus cereus)ZJB-07112酰胺酶的分离纯化及其酶学性质[EB/OL]
北京：中国科技论文在线
） 摘要： 用一株蜡状芽孢杆菌新菌株ZJB-07112 (Bacillus cereus ZJB-07112)发酵生产酰胺酶
） Abstract： A amidase from a strain Bacillus cereus ZJB-07112 was purified to homogeneity by using of the sonication, anion-exchange chromatography, Pheny1-Sepharose chromatography
The molecular weight of amidase was estimated to be 60
6 kD by 12
5% SDS-PAGE
Its N-terminal sequence was ATIRPDDKAI
The optimum temperature and pH of the amidase for acrylamide were pH 7
5 and 35 ℃, respectively
The enzyme was unstable at temperature over 50 ℃ and only 10
8% activity was retained after exposure to 60 ℃ for 30 min
Most of metal ions and EDTA had no significant effect on the enzyme activity, whereas Hg+
酰胺酶
该酶在50 ℃以上容易失活
（ 1、 浙江工业大学生物工程研究所
2、 浙江工业大学生物工程研究所
Ag2+ and urea caused obvious inhibition
The Km and Vmax values of amidase for acrylamide were 2
64 mmol/L and 0
6 μmol/(min•ml), respectively
Keywords： Bacillus cereus
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