16S rRNA克隆文库法分析成人龋病唾液微生物多样性[EB/OL]
北京：中国科技论文在线
构建16S rRNA克隆文库
高龋组中优势菌（链球菌属、普氏菌属、颗粒链球菌属）对龋病发生发展的作用还有待进一步的研究
CA组优势属为：链球菌属（Streptococcus,53
16%）、普氏菌属（Prevotella,28
77%）、颗粒链球菌属（Granulicatella,9
34%）
CF组优势菌为：链球菌属（Streptococcus,46
12%）、普氏菌属（Prevotella,23
41%）、奈瑟菌属（Neisseria,14
35%）
） Abstract： Objective: The aim of this study is to discuss the difference of saliva microbial structure between the caries-active and caries-free population
Methods: According with WHO sampling standard, 6 saliva samples were collected with 3 caries-active(CA) and 3 caries-free(CF)
Extracting the saliva sample's DNA and constructing the 16S ribosomal RNA gene clone library
The positive clones were selected to sequences and analysis by software such as MOTHUR, etc
The phylogenetic tree was built by MEGA 4
0
Results: A total of 87 OTUs were obtained and all of the OTUs were classed 5 phyla, 9 classes, 10 orders, 14 families, 19 genera
The dominant genera in CA included: Streptococcus(53
16%), Prevotella(28
77%), Granulicatella(9
34%); The dominant genera in CF included: Streptococcus(46
12%), Prevotella(23
41%), Neisseria(14
35%)
Conclusion: As 16S ribosomal RNA gene clone library method has already matured, it can be used to study oral microbial community structure
There are obviously differences between the Han caries-active and caries-free population
The specific mechanism of the dominant genera in CA remains further research
Keywords： Oral microorganisms; Oral cavity; Dominant bacteria; 16S rRNA; Clone library
下载PDF阅读器 PDF全文下载： 修改稿 1（ 0 ） 初稿 （ 13 ） 作者简介： 李俊平（1989-）
当地汉族人群中高龋和无龋人群口腔微生物群落结构存在一定的差异
