甘蓝花粉蛋白（BoEF-Tu）的克隆及其序列分析[EB/OL]
北京：中国科技论文在线
牛义 1
SONG Ming 3, （ 1、 College of Horticulture and Landscape Architecture, Southwest University, ChongQing 400715
主要研究方向：园艺植物遗传育种与生物技术 【收录情况】 中国科技论文在线： 王志敏
） Abstract： In this paper,the elongation factor BoEF-Tu was isolated and characterized from Cabbage (Brassica oleracea L
var
capitata) line E1 using RT-PCR and RACE-PCR
The Cabbage Botuf cDNA clone contained 1 728 bps with an open reading frame（ORF）located from 112 to 1 473 bp, encoding a protein of 453 amino acids
The predicted molecular mass of BoEF-Tu was 49
17 kD with acidic pI of 6
52
Bioinformatic analysis results showed that BoEF-Tu protein might contain 3 conserved domains and 9 active site motif, but had no signal peptide sequences and transmembrane region
Homology analysis of the deduced amino acids indicated that the sequences had 93% similarity with Arabidopsis thaliana EF-TuM
The phylogenetic tree showed that the BoEF-Tu had the closest genetic relationship with Arabidopsis thaliana EF-Tu
Keywords： vegebable; cabbage; pollen; elongation factor 下载PDF阅读器 PDF全文下载： 初稿 （ 90 ） 作者简介： 王志敏
BoEF-Tu与拟南芥EF-TuM同源性较高
