Wang Ling 1, （ 1、 Life Science College, Anhui Normal University, The Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, 241000, China
2、 The Provincial Key Lab of the Conservation and Exploitation Research of Biological Resources in Anhui, Wuhu, Anhui, 241000, China
聂刘旺 2
） Abstract： Ten polymorphic microsatellite loci were developed for the Chinese Pond turtle (Mauremys reevesii) from (AC)n and (GATA)n microsatellite-enriched genomic library
We screened 32 wild individuals from the middle and lower reaches of Changjiang River and detected high levels of polymorphism for all ten loci
The number of alleles/locus ranging from 3 to 18 with the values of expected and observed heterozygosities ranging from 0
585 to 0
907 and 0
469 to 1
000, respectively
Cross-species amplification of these microsatellite loci was also tested in additional seven freshwater turtle/half freshwater turtle species (Mauremys megalocephala, Cuora galbinifrons, Cuora flavomarginata, Mauremys mutica, Mauremys sinensis, Pelochelys cantorii, Rafetus swinhoei) of Cryptodira
All 10 loci were ampli?ed speciffc products in the five species of Geoemydidae, while in the P
cantorii and the R
swinhoei of Trionychidae only one locus and two loci worked, respectively
Therefore, these highly variable loci may serve as a valuable tool for population genetics analyses, gene flow and provide information on the evolutionary history of the species
Keywords： Mauremys reevesii; Microsatellites; Genetic diversity; Conservation; Cross-amplificatio????? 下载PDF阅读器 PDF全文下载： 初稿 （ 139 ） 作者简介： 刘罗,男,硕士研究生,主要研究方向为分子生态学 通信联系人： 聂刘旺,1962年生,男,博士,教授,博士生导师 【收录情况】 中国科技论文在线： 刘罗
卜兴江 1
） 摘要： Ten polymorphic microsatellite loci were developed for the Chinese Pond turtle (Mauremys reevesii) from (AC)n and (GATA)n microsatellite-enriched genomic library
We screened 32 wild individuals from the middle and lower reaches of Changjiang River and detected high levels of polymorphism for all ten loci
The number of alleles/locus ranging from 3 to 18 with the values of expected and observed heterozygosities ranging from 0
585 to 0
907 and 0
469 to 1
000, respectively
Cross-species amplification of these microsatellite loci was also tested in additional seven freshwater turtle/half freshwater turtle species (Mauremys megalocephala, Cuora galbinifrons, Cuora flavomarginata, Mauremys mutica, Mauremys sinensis, Pelochelys cantorii, Rafetus swinhoei) of Cryptodira
All 10 loci were ampli?ed speci?c products in the five species of Geoemydidae, while in the P
cantorii and the R
swinhoei of Trionychidae only one locus and two loci worked, respectively
Therefore, these highly variable loci may serve as a valuable tool for population genetics analyses, gene flow and provide information on the evolutionary history of the species
关键词： Mauremys reevesii; Microsatellites; Genetic diversity; Conservation; Cross-amplificatio Liu Luo 1,
